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2.5.6.6.1 Asymmetric Direct Reductive Amination of Ketones with Ammonium Formate

DOI: 10.1055/sos-SD-202-00095

Xu, L.; Wu, X.; Xiao, J.Science of Synthesis: Stereoselective Synthesis, (20112303.

The ω-transaminase protocol employs three enzymes in one pot, which function cooperatively in the asymmetric direct reductive amination: (1) an ω-transaminase ATA-113, which transfers the amino group from alanine to the substrate ketone, affording the desired amine and pyruvate; (2) alanine dehydrogenase (ADH), which regenerates alanine from pyruvate by consuming ammonium and NADH; (3) formate dehydrogenase (FDH), which recycles the NADH cofactor from NAD+ and formate (Scheme 52).[‌193‌]

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