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1.4.5.5.2 Enzyme Engineering of Hydantoinases and Related Enzymes

DOI: 10.1055/sos-SD-214-00283

Slomka, C.; Engel, U.; Syldatk, C.; Rudat, J.Science of Synthesis: Biocatalysis in Organic Synthesis, (20151407.

Initially, hydantoin-hydrolyzing enzymes must be screened and isolated. After the optimization of reaction conditions and investigation of biochemical characteristics, enzyme engineering allows the alteration of intrinsic properties of promising enzymes. By recombinant DNA technology and directed evolution, the catalytic activity, substrate specificity, stereospecificity, stability, and the expression level of enzymes can be improved. Moreover, the elucidation of reaction mechanisms and the 3D structure of catalysts enables a rational, structure-based design of promising enzymes.[‌37‌]

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