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2.3.1.3.1.1 Proteins Expressed Separately

DOI: 10.1055/sos-SD-215-00109

Faber, K.; Hall, M.Science of Synthesis: Biocatalysis in Organic Synthesis, (20152237.

The use of an external regeneration system based on a recycling enzyme expressed separately from the biocatalyst has the advantage of being simple to handle. The activity of the regeneration system can be easily tuned by adjusting the amount of protein required to achieve efficient nicotinamide regeneration, independently of the reducing activity of the ene-reductase (Table 9).[‌38‌,‌39‌,‌52‌,‌64‌,‌84‌]

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Meeee Meeeeeeee Meeeeee Meeeeeee Meeeeee Meeeeeeeee (%) ee (%) Mee
8 MeeM MMM/eeeeeeee (8 eeeee) >88 >88 (M) [‌88‌]
8 MMM8 M8MMM/eeeeeee 8-eeeeeeeeee (8 eeeee)  88  88 (M) [‌88‌]
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8 MMM MMM-M/eMeMMe (8 eeeee)  88  88 (M) [‌88‌]
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M8 M8 M8 Meeeeeee Meeeeeeeee (%) ee (%) Mee
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M Me Me MMM8/MMMM 88 88 (M) [‌88‌]
M Me M Mee/MMMM 88 [‌88‌]
M Me Me Mee/MMMM 88 88e [‌88‌]

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References


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