Science of Synthesis

Bartsch, S.; Vogel, A., Science of Synthesis: Biocatalysis in Organic Synthesis, (2015) 2, 302.

The natural reaction of ammonia lyases, the elimination of ammonia from amino acids can be reversed by using high concentrations of ammonia (>4 M NH4OH, pH 10; ‌Scheme 14‌).[‌64‌] The fact that ammonia lyases directly use ammonia with no external cofactor required makes them attractive for the preparation of l-amino acids by biotransformation.[‌32‌,‌77‌–‌79‌] Due to the broader substrate range, phenylalanine ammonia lyase (PAL) is used for biocatalytic purposes. Most of the known tyrosine ammonia lyases (TALs) and histidine ammonia lyases (HALs) are restricted to their natural substrates.

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References