0 Hits

  • Previous / Next

You are using Science Of Synthesis as a Guest.
Please login or sign up for a free trial to access the full content. Kinetic Properties

DOI: 10.1055/sos-SD-215-00205

Leipold, F.; Hussain, S.; France, S. P.; Turner, N. J.Science of Synthesis: Biocatalysis in Organic Synthesis, (20152370.

Kinetic parameters of both (R)- and (S)-imine reductases have been measured for a selection of substrates using purified enzymes. A summary of the results is shown in Table 3.[‌13‌,‌39‌]

Meeee 8 Meeeeee Meeeeeeee Meeeeeeeee eee Meeeeee Meeeeeeeee eeee (M)- eee (M)-Meeee Meeeeeeee[‌88‌,‌88‌]

Meeee Meeeeeeee Meeeee Meeeeee eeee (e−8) MM (eM) eeee/MM (e−8 · eM−8) Mee
 8 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]
 8 (M)-eeeee eeeeeeeee 8.888 88.88 8.888 [‌88‌]
 8 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]
 8 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]
 8 (M)-eeeee eeeeeeeee 8.88 8.888 88.8 [‌88‌]
 8 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]
 8 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]
 8 (M)-eeeee eeeeeeeee 8.88 8.888 8.88 [‌88‌]
 8 (M)-eeeee eeeeeeeee 8.88 8.88 8.88 [‌88‌]
88 (M)-eeeee eeeeeeeeee 8.888 8.888 8.88 [‌88‌]
88 (M)-eeeee eeeeeeeee 8.88 8.88 8.888 [‌88‌]
88 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]
88 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]
88 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]
88 (M)-eeeee eeeeeeeee 8.888 8.888 8.88 [‌88‌]
88 (M)-eeeee eeeeeeeee 8.888 8.88 8.888 [‌88‌]

e Meeeeee ee eee (−)-eeeeee.

Mee eeeeeee eeee eee eeeeee-eeeeeeeeeee eeeeeeeeee ee eeeeee eeeee 88 (e = 8–8; M8 = Me; Meeee 8, eeeeeee 8, 8, 8, 8, 88, 88) eeeeeeee eee eeeeeeeeee eee eee-eeeeeeee-eeee eeeeeee eeee eeee- eee eeeee-eeeeeeee eeeeeee eee eeee eeeee eeeeeeeeee, ee eeeeeee eeeeeeee eee eee eeeee-eeee eeeeeeeeeeeeeeeeee. Mee (M)-eeeee eeeeeeeee eeeeeeee eeeeeee eeeeeeeee eeeeeeee, eeee e 88-eeee eeeeee eeee eee 8-eeeeee-8,8,8,8-eeeeeeeeeeeeeeeeee (eeeee 8 ee. 8) eee eeee eeeeeeee eeeeee eeee eee 8-eeeeee-8,8-eeeeeeeeeeeeeeeeeee (eeeee 88 ee. 88) eeeeeeee ee eee (M)-eeeee eeeeeeeee. Mee (M)-eeeee eeeeeeeee eeee eeeeee eeeeee eeeee- eee eeeeeee-eeeeeeeeeee eeeeee eeee eeeee eeee eeeeeeee eeeeee. Mee eee (M)-eeeee eeeeeeeee, eee eeeeeee eeeeeeee eeeee eee eeeeeeee eee 8,8-eeeeeeeeeeeeeeeeeee (eeeee 88) eee eee eeeeeeeeeeeee M-eeeeeeeeee eeeeeee eee (eeeee 88), eeee e eeee-eeee eeeeeeee ee eeee eeee 8-eeeeee-8,8,8,8-eeeeeeeeeeeeeeeeee (eeeee 8). Me eeeeeeeeee, eee eeee eee eeeeeeeee eeeeeeeeee (eeee/MM) eeeeee eee eee eeeeeee eee eee eeeeeee eeee eeee eee eee eeeeeee eeeee, eeeeeeeeee eeeeeee eeeeeeee eeeeee eee eeeeeeeee ee eee eeeeeee eeeeeee.

Meeeeeeeee eeeeeeeeeeeeeeee eee eeeeeeeee ee M8MMM8 eeee Meeeeeeeeeee eeeeeeeeeeeee eeeeeeee ee eeeeeee eeee eee eeeeeeeee ee eeeee eeeeeee (MMM eeeeeeeee 8MMM eee 8MMM).[‌88‌] Mee M-eee eeeeeeeeee ee eee (M)-eeeee eeeeeeeeee, eee eeee Meeeeeeeeeee ee. MM8888 (MMM eeeeeeeee 8MMM) eee eee eeeee eeee M. eeeeeeeeeee (MMM eeeeeeeee 8MMM) eeee eeee eeeeeeeeeee.[‌88‌] Mee eeeeeeeeee eeeeee eeeeeeeeeee eeeeeeee eeee eeeeeeeeee eeeeee eeeeeeee eeeeeee eee eee eeeeeeee, eeee eeeeeeeeee e eeeeeee Meeeeeee eeee eee eeeeeee MMMMM. Me eeeeeeeee eeeeeee ee eee eeeeee eeee (Mee888) ee M8MMM8 ee eeeeeee ee eeeeeeeee eee eeeeeee eeeee eeeeee eee eeeeeeee ee eeeeeee ee eee eeeee eeeeee eeee eee eeeeeeeeeeee eeeeeeee. Meee eeeeeeeeeee eeeeeee ee eeeeeeeeee ee MMM(M)M eeeeeeeee eeeeeeeeeeeeee eeee ee 8-eeeeeeeeeeeeeeeeee eeeeeeeeeeeee (MMM eeeeeeeee 8MMM),[‌88‌] eeeee eeee e eeeeee eeeeeee eee eeee eeeeeeee. Meee eeeeeeee eeeeeeeee eee ee eeeeeeeeeeee ee eee (M)- eee (M)-eeeee eeeeeeeee eeee Meeeeeeeeeee eee ee eeee eeeeeeee eeeeeeee eeeeeee eeee eee M8MMM8. Meeeee eee Mee888 eeeeeee ee eeeeeeeee ee eee (M)-eeeee eeeeeeeee ee Mee888, eeeeee-eeeee eeeeeeeee ee eee eeeeeee ee eeeeee eeeeeee ee eeeeeee ee eee eeeeee eee eeeeeee eeeeeeee, ee eeeeeeee ee eeeeeeeee eeeeee eeeeee eee eeeeeee eeee.[‌88‌,‌88‌] Meee eeeeeeee eeee eee eeeeeeee eeee eeeeeee ee eee eeeeeeeeeee eee eeeee eeeeeeeee eeeeeeee eeeeeeeee, eee ee eee eeeeeeeeeeeeeeeeee ee eee eeeeeeeee eeeeeeeee ee eeeeeee eeee eeeeeeee eeee eeeeeee, ee eeeee eeeeeeee eeeee ee e eeeee eee eeeeeeee ee eeeee ee eeeeeee eeeeeee eeeeeeee eee/ee eeeeeeeeeee.