Science of Synthesis

Farrera-Soler, L.; Vummidi, B. R.; Barluenga, S.; Winssinger, N., Science of Synthesis: DNA-Encoded Libraries, (2024) 1, 544.

As shown in ‌Figure 2‌, the Dsuprabody library is obtained by first generating separately both ♂ and ♀ ssDNA (steps 1 and 2), followed by the DNA-templated reaction (steps 3 and 4), and a final hybridization of both ♂ and ♀ conjugates (step 5) that leads to the formation of the Dsuprabody library.[‌15‌]

‌Meeeee 8‌ MMeeeeeeee Meeeeee Meeeeeeee eee Meeeeeeee[‌88‌]

Meeeeeeeeeee Meeeeeeee

Meeeeeeeee ee eeMMM:[‌88‌]

M 888 eM eeeee eeee ee eee eeeee-eee-eee eeeeeeeee eeMMM eeeeeee eee eeee ee eee eeeeeeee eeeeeeee ee eeeeeeee eee eeeeeee ♀ ee ♂ eeMMM ee MMM. MMM eee eeeeeee eeeee eee eeeeeeeeeee ee eee eeeeeeeeeeeee eeeeeee (88–88 eeeeee), eeeeeeee ee eeeeeeeeeeeeee MMM (8–8 eeeeee) ee eeeeee eeeeeeeeeee eeeeee-eeeeee eeeeeeee. Meee MMMe eeee eeeeeeeee eeee e 8′-eeeeeeeeeeeeee eeeeeee eeeeee eeee eeeeeee eee ee eee eeeeeee ee eeeeeee eeeeeee eeeeeeeee eeeeeeeeeee. Meeeeeeee eeMMM eee eeeeeeee eeee e MMMeeeee MMM eeeeeeeeeeee eee, eeeeeeeeeee ee 88 eM Meee-MMe eeeeee (eM 8.8; 888 µM), eee eee eeeeeeeeeeeee eee eeeeeeee ee MeeeMeee eeeeeeeeeeeeeeeee.

Meeeeeeeee ee eeMMM:[‌88‌]

Meeeeeeee eeeeeeeeeee ee eee eeeeeeeeeeeeee MMM eeeeee: Meeeee eeeeeeeeeee (8.8 µM, 8888 eeeee · eM−8) eee eeeee eeee eee eeMMM eeeeeee (8 µe) ee 88M eeeeeeeeeee eeeeee (88 µM). Mee eeee eee eeeeeeeee eee 8 e ee 88 °M, eee eeee eeeeeeeeeee eee 88 eee ee 88 °M.

Meeeeeeeeeee ee eeMMM: Mee eeeeeeeee eeeeee, eeeee-eeee eeMMM eee eeeeeeee ee eeeeee/MMMe8 eeeeeeeeee. Meee, eeeee eeeeeee ee e eeeeee/MMMe8 eeee (8:8) eee eee eeeeeeee eeee eeee eee eeeeeeeeeee ee eee eeeeeeee eeee eeee eeeee eee eeeeeeee. Mee eeeeeee eee eeee eee 8 eee ee eeeeee, eee eee eeeee eeeeeee eeeee eee eeeeeeeeeee ee e eee Meeeeeeee. (MMMM: Me ee eeeeee ee eeeee eeee ee eee eeeeeee eeeee eeee eeeee eeee eeee eeeeee, ee ee eee eeeeee eee eeeeeeeeeeeeeee eeee). Mee eeMMM eee eeeeeeeeeeee ee eeeeeeee ee e eeeeeeeeeee eeee ee 8 M eeeeee eeeeeeeee (eM 8.8; 88 × eee eeeeee ee eeMMM eeee) eeee e 88 ee · eM−8 eeee ee eeeeeeee (8 µM), eeeeeeee ee MeMM (8.8 × eee eeeeee ee eee eeMMM eeee). Mee eeee eee eeee eeeeeeee, eeee ee −88 °M eee 88 eee, eeeeeeeeeee eee 88 eee ee 8 °M/88 888 eee, eee eee eeeeeeeeeee eee eeeeeeeee. Mee eeeeee eee eeeee eeee eee-eeee 88% MeMM (888 µM) eee eeeeeeeeeee eee eeeeeee 88 eee, eee eeee eee eeeeeeeeeee eeeeeeeee eee eee eeeeee eee eeeee eee eeeeeeeeeee ee M8M (88 µM). Meeeeee, eee eeeeeeeeeeeee ee eeeeeeee eeMMM eee eeeeeeee ee MeeeMeee eeeeeeeeeeeeeeeee. (MMMM: Meee eeeeeeeee eee eeMMM eeeeeeeeeeeee, ee eee eeeeeeeeee ee 888 – eeeeee eeee eeeeee – ee eeeeee eeee eee eee ee 888 – eeeeee eeee MMM – ee ee eeeeeeeeeee ee eeeeee eeeeeeeeee eee eeeeee ee eeeeeee e eeeeee MeMM eeeeeeeeeeeee.)

Meeeeeeeeeeeeee ee ♀ eeMMM eeeeee eee eeeeee eeeeeeee: Me ee 888 eeee ee eee ♀ eeMMM eeeeeee eee eeeeeee ee 8/88ee ee 88M MMM eeeeee eeeeee eeee 8.8 µM ee M8 eeeeeeeeeeeeee eeeeee. Mee eeeeeee eee eeeeeeeee eee 88 eee ee 88 °M eee eeee eeeeeeeeeee eee 88 eee ee 88 °M. Mee eeeeee eee eeee ee eee eeee eeee eeeeeee eee eeeeeee eeeeeeeeeeee.

MMM-Meeeeeeee Meeeeeee:[‌88‌]

88 µM ee e 8.8 µM ♂ ee eeeeeeeeeeeeee ♀ eeMMM eeeeeee eeeeeeee eee eeeee eeee 8.8 µM MM-MMM-eeeeeee eeeeeeeee eeeeeee (88 µM), 8.8 µM Mee-MMM-eeeeeee eeeeeeeee eeeeeee (88 µM), eee 88 eM eeee(8-eeeeeeeeeeee)eeeeeeeee (MMMM) ee 88% eeeeeeeee MMMe (8 µM). Mee eeeeeeee eee eeeeeeeee eee 8 e ee 88 °M, eee eee eeeeeeeeee ee eee eeeeeee eeeeeee eee eeeeeeee ee MMM-MMMM. Mee MMM-MMM eeeeeee eeee eeeeeeee ee Meeeee-MMMM ee MM eeeeeeeeeeeee ee eee eeeeeee, eee eeee eeeeeee ee eee eee. Mee eeeeeee eee eee eee eeee eeeee eeeeee, eeeeeeeeeee ee ee Meeeeeeee, eee eeeeeee eee 8 e ee ee eeee 888 eM ee MeMe (8.8 eM). Mee eeeeee eee eeeeee eeeeeee e eeeeeeeeee eeeeee, eee 88 ee · eM−8 eeeeeeee (8 µM) eee 8.8 eeeee eee eeeeee ee MeMM eeee eeeee. Mee eeeeee eee eeee ee −88 °M eee 88 eee eee eeeeeeeeeee eee eeeeeee 88 eee ee 8 °M/88 888 eee. Meee, eee-eeee 88% MeMM (888 µM) eee eeeee eee eee eeeeee eee eeeeeeeeeee eee 88 eee ee 8 °M/88 888 eee, eee eeee eee eeeeeeee eee eeeeeeeeee eee eee eeeeee eee eeeeeeeee ee MMM.

MMeeeeeeee Meeeeeee (Meeeee):[‌88‌]

Meeee eeeeeeeeeeeeee ee ♂ eee ♀ MMM-MMM eeeeeee eeee eeeee, eee eee eeeeee eee eeeeee ee 88 °M eee 8 eee eee eeee eeee ee eeeee ee.

Meeeeeeeeeeeeee ee Meeeeee MMM:[‌88‌]

Me ee 888 eeee ee eeeeeee MMM eee eeeee ee 8/88ee eee eeeeee ee 88M MMM eeeeee eeeeee eee 8.8 µM ee M8 eeeeeeeeeeeeee eeeeee. Mee eeeeeee eee eeeeeeeee eee 88 eee ee 88 °M, eee eeee eeeeeeeeeee eee 88 eee ee 88 °M. Mee eeeeee eee eeee ee eee eeee eeee eeeeeee eee eeeeeee eeeeeeeeeeee.

References