Science of Synthesis

Farrera-Soler, L.; Vummidi, B. R.; Barluenga, S.; Winssinger, N., Science of Synthesis: DNA-Encoded Libraries, (2024) 1, 546.

The reported screening is based on three selection rounds, with increased stringency in each round. Nonetheless, depending on the characteristics of the protein, as well as on the expected affinity of the library members, the stringency of the selection (quantity of protein vs. library, number/temperature/duration/buffer of washes, or the number of rounds of selection) may need to be adapted to obtain a more informative result.[‌15‌]

Meeeeeeeeeee Meeeeeeee

Meeee Mee ee Meeeeeeee:[‌88‌]

M888 Meeeeeeeeeee Meeeeeeee (88 µM) eeee eee eeeeeee eeeeeeeeeee eeeeeee eeee eeeeee eeeee eeee MMMe (8 eM eee eeee eeee 888 µM). Meee, 888 µM ee eee Meeeeeeeee eeeeeee ee MMMe eee eeeee ee eee eeeee eee eeeee eee 88 eee. Mee eeeeeeeeee eee eeeeeeeeeee ee e eee Meeeeeeee, eee eeeeeeeeeee eee eeeeeee (eeee eee eee ee e eeeeee), eee eee eeeee eeee eeeeee eeee eeeee eeee MMMe (888 µM eee 8 eee eee eeee eeee; eeeeee eee eeeee eeee, eee eeeeeeeeee ee eee eeeee eee eeeeeeeeeee eeee e eee Meeeeeeee ee eeeeee eeeeee eeeee ee eee eeeee ee eee eeee). Mee eeeeeeee ee eee eeeeeeee ♂ eee ♀ MMM eeeeeee eee eeeeeeeee eeee eee eee ee eee eeeeeeeeeeeeee eeeeeee ee eeeeee 88 µM ee MMM eeeeee eeeeeeee (88 µM eeeeeee eeeeee, 8 µM ee 88M MMM eeeeee eeeeee, 8 µM ee 8.8 eM eeeeeeeeeeeeee eeeeeee, eee 8 µM ee MMM eeeeee) eee eeeeee eee eeeeeeeeee eee 88 eee ee ee. Mee eeeee eeee eeee eeeeee ee 88 °M eee 8 eee, eee eee eeeeeeee eeeeeeeeeee (eee eeeeeeee eeee eee eeeeeeeeeee) eeee eeeeeeee eeee e MMM eeeeeeeeeeee eee eeeee ee eee eeee eeeee ee eeeeeeeee ee eeeeeee.

Meeeee Mee eee Meeee ee Meeeeeeee:[‌88‌]

Mee eeeeeeee eeeeee eeeeee (8–8 µM) eee eeee ee eeeeeeee e eee Meeeeeeeee eeeeeee, eeeeeeee eeee eee eeeee eeeeeeeee ee Meeeeee ‌8.8.8.8.8.8‌. Mee eeee eeeeeeeeee eeeeeee eee eeeee eee eeeeeee eeeeee ee eeeeeeeee eee eeeeeeeee eeeeeeeeee, ee eeeeeeee eee eeeeee ee eeeee (eeee 88 µM ee eee eeeee eeeee ee 88 eee 88 µM ee eee eeeeee eee eeeee eeeeee, eeeeeeeeeeee) eee ee eeeeeeeeee eee eeeee eeee 8%, 8.88%, eee 8.88% ee MMM eee eee eeeee, eeeeee, eee eeeee eeeeee ee eeeeeeeee, eeeeeeeeeeee.

References