Navigation

0 Hits

  • Previous / Next

You are using Science Of Synthesis as a Guest.
Please login to access the full content or check if you have access via
1.3.3.4.2.1 Synthesis of Phosphorylated Nucleosides

DOI: 10.1055/sos-SD-214-00162

Wever, R.; Babich, L.; Hartog, A. F.Science of Synthesis: Biocatalysis in Organic Synthesis, (20151234.

In the past, the major methods for the large-scale production of nucleotides were chemical phosphorylation of the corresponding nucleosides,[‌72‌] direct fermentation to give inosine 5′-monophosphate (1), enzymatic amination of xanthosine 5′-monophosphate, phosphorylation of inosine by a guanosine/inosine kinase using whole cells,[‌73‌] or an enzymatic procedure with inosine kinase from Escherichia coli as a phosphorylating enzyme.[‌74‌] The kinase requires adenosine triphosphate (ATP), which needs to be regenerated, making the process more complex. Because of this, and the large market for inosine 5′-monophosphate as a flavor enhancer, considerable effort has been put into optimizing the direct enzymatic phosphorylation of nucleosides by pyrophosphate.

Me eeeeeee[‌88‌] eee eeeeeeeeee ee eeeeeee 8′-eeeeeeeeeeeee (8), eeeeeeeeeeee eeee eeeeeee eeeeeeee eeee eeeeeeeeeeee[‌88‌,‌88‌] eee e eeeeeeee eeee eeeeeeee eeeeeeeeeee eeeee[‌88‌] eee eeeeeee eee ee eee eeeeeeeeeee eeee Meeeeeeeeee eeeeeee. Mee eeeee eeeeee eeeeee eeeeeeee eee eeee ee eeeeeee 888 e · M−8 ee eeeeeee 8′-eeeeeeeeeeeee (8) eeee e eeeee eeeee ee 88% eeee eeeeeee.[‌88‌,‌88‌] Mee eeeeeeeee eeeeeeeeeeee eeeeeeee eeeeeee ee e eeeeeeee ee eee Meeeeeeee eeeeeeee (Me) eeeee eee eeeeeee, ee, eeeeeeeeeeeee, eee eeeeeeeeee ee eeeee ee eee eeeeeee–eeeeee eeeeeeeeeeee ee eeeeeeeeee. Meee eee eeeeeee eeeee eeeeeeeeeeeee ee eeeee ee eeeeee, eeeeeeeeeee, eee eeeeee eeeee eeee eeeeeeee eeeeeeeeee. Meeeeeeeeeeee eee eeeeeeeee eee eeeeeee eee ee e eeeeeeeeee eeeee eeeee eee ee eeeeeeee eeeeee eee eeee eeeeeeee. Meee eeeeeeee, eeeee eee eeeeee eeeeee eeee M. eeeeeee[‌88‌] eee eeeeeeee ee Meeeeʼe eeeee eeee eeeeeeeee eee 88 eM ee eeeeeee eee eeee eeeeeeeee eeee 88.8 eM ee eeeeeee 8′-eeeeeeeeeeeee (8). Mee-eeeeeeee eeeeeeeeeeeeee ee eeee ee eeeeeee eeeeeee 8 (88% eeeee).

Meeeeeee eeeeeeeeee ee eee eeeeee eee eee eeeeee eeeeeeeeeeeeee eeeeeeee 8 eeee eeeeeee eeeeeeeeee ee eee eeeeee, eee e eeeeee eeeeeeeeee-eeee eeeeee-eee eeeeeee eee eeee eeeeeeeee eeeee eeeeeeee eeee eeeeeeeeeee eeee Meeeeeee eeeeeeee (MeeM-Me) eeeeeeeeeee ee Meeeeeeee (Meeeee 8).[‌88‌] Meee eeeeee eee e eeee eeeee eeeeeeeee eeeeeeeeee eeeee, ee eeeeeeeeeeeee (eeeeeeeeeeeeee eeee eeeeeee eeeeeeee), eee ee eeee eeeeee eeeee eeeeeeee.

Meeeee 8 Meeeee-Mee Meeeeee Meeeeeeeee Meeeeeeeeee Meee Meeeeeeeeee[‌88‌]

Me eeeeee eee eeee ee eeee ee eee eeeeee eee eee eM ee ee eeeeeeee ee eeeeeeee eee eeeee ee eee eeeeeeeeeeeeeee eeeeeee. Mee eeeeeeeeee eeeeeeee ee eeee eeeeee ee eeeeeeeeeeee eeeee eeee eeee eeeeeeee eeeeeee,[‌88‌,‌88‌,‌88‌] eee ee eeeeee ee eeeeeeeeee eeee eee MeeM-Me eee eee eeeeeeeee eee eeee eeeeeeee.

Meeeeeeeeeee Meeeeeeee

Meeeeee 8′-Meeeeeeeeeeee (8); Meeeeee Meeeeeeee Meeee e Meee Meeeeee:[‌88‌]

M eeee eee eeeeeeee eeeeeeeeee 888 eM eeeeeee eee eeeeeeeeeeeee (888 eM), eeeee eee eee ee eM 8 ee eeeeee eeeee ee Me8M8M8M8 eee Me8M8M8. Meee eee eeeeee (8.88 eM · eee−8) eeeeeee e 8.8-eM eeeeee eeeeeeeeee eeee eeeeeeeeeee eeee Meeeeeee eeeeeeee (MeeM-Me) eeeeeeeeeee ee Meeeeeeee. Meeee 8 e, e eeee (888 eM) eee eeeeeeee eeeeeeeeee 88 eM eeeeeee 8′-eeeeeeeeeeeee (8), 88 eM eeeeeee, eee ee. 888 eM eeeeeeeee. Mee eM ee eeee eeee eeeeeeeeee eeeeeee, eee eeeeeee eeeeeee 8, eee eeeeeeeee eee eee ee 8. Me eeee eM eeee ee eee eeeeeeeee eeeeeeeeeeee eee eee eeeeee eeeeeee eeee eee eeee. Meeeeeeeeeee, MMMM-Meeeeeee M88 (88 e) eee eeeee ee eee eeee, eeeee eee eeee eeeeeee. Mee eeeee eee eeeeee eeee e eeeeee eee eeeeeee 8′-eeeeeeeeeeeee (8) eee eeeeee eeee e eeeeeeee ee MM8MMM8 (eM 8.8; 88, 888, eee eeee 888 eM). Meeeeeeee eeeeeeeeee eeeeeee 8 eeee eeeeeeeee eee M8M eee eeeeee eeee eeeeeee ee eeeeee-eeeeee; eeeee: 8.8 e (88–88% eeeeeeee eeeee). Mee eeeeeee eee eeee eeeeeeeee ee MMMM eee MM/eee eeeeeeeeeeee.

References


Cookie-Einstellungen