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DOI: 10.1055/sos-SD-214-00283

Slomka, C.; Engel, U.; Syldatk, C.; Rudat, J.Science of Synthesis: Biocatalysis in Organic Synthesis, (20151405.

A dihydropyrimidinase has been successfully used for the conversion of nonnatural 6-monosubstituted dihydrouracils to develop a potential tool for the production of β-amino acids.[‌161‌] The dihydropyrimidinase from Sinorhizobium meliloti CECT4114 shows hydantoinase as well as dihydropyrimidinase activity, while the natural six-membered-ring compounds are more suitable as substrates. The β-ureidopropionase from Agrobacterium tumefaciens C58 has also been characterized. This enzyme has a broad substrate spectrum hydrolyzing unsubstituted N-carbamoyl-α-, -β-, -γ-, and -δ-amino acids as well as several monosubstituted substrates. Therefore, it is also of industrial interest in terms of β-amino acid synthesis.[‌83‌]

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e-α-Meeeee-β-eeeeeee (e-88); Meeeeee Meeeeeeee Meeee Meeeeeeeeeeee Meeeeeeee eee Meeeeeee Meeeeee:[‌888‌]

Mee eeeeeeeeeee M. eeee MM88-Meee eeeeeee eeee eeeeeeeeee ee eeeeeeee eeeee eeeeee (8888 eM) eeee eeeeeeeeee (888 µe · eM−8) ee 88 °M. Meeee ee eeeeeeeeee eeee ee 8–8 e eee ee ee MM ee 8.8–8.8, eeeeeeee (8.8% e/e) eee eeeee eee eeeeeeeeee eeeeeeeee. Mee eeeeeeeeeee eee eeeeeeeee ee 88 °M eeeeeeeee eee eee eeeee eeee eeeeeeeeeeee eeeeeeeee ee eeeeeeeeeeeeee (8888e, 88 eee, 8 °M). Meeeeeeeeeee eee eeeeeeeeeee ee eee eeeeeee eee eeeeeee eee ee eeeeee eeeeeeee eeeeeeeeeeeeee. Me eeeeeee eeeeeeeee ee MMM ee eeeeeee, e eeeeeeeeeeee eee-eeeeeeeeee eeeeeeeeeeeeee eeee eee eeeeeeeee ee MMMM eeee e Meeeeeee 888 eee eeeeeeeeee eeeeee. Mee eeeeeeeeeeeee ee eee eeeeeeee eeeeeee, eeeeeeee eeeeeee 8.8 M eeeeee eeeeeeeee eeeeee (eM 8.8) eee eeeeeee eee eeeee eeeeeeee eeeeeeeeeeeee (Meeeeeee, Meee, Meeeeíe, Meeee).

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