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DOI: 10.1055/sos-SD-215-00109

Faber, K.; Hall, M.Science of Synthesis: Biocatalysis in Organic Synthesis, (20152237.

The use of an external regeneration system based on a recycling enzyme expressed separately from the biocatalyst has the advantage of being simple to handle. The activity of the regeneration system can be easily tuned by adjusting the amount of protein required to achieve efficient nicotinamide regeneration, independently of the reducing activity of the ene-reductase (Table 9).[‌38‌,‌39‌,‌52‌,‌64‌,‌84‌]

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Meeee Meeeeeeee Meeeeee Meeeeeee Meeeeee Meeeeeeeee (%) ee (%) Mee
8 MeeM MMM/eeeeeeee (8 eeeee) >88 >88 (M) [‌88‌]
8 MMM8 M8MMM/eeeeeee 8-eeeeeeeeee (8 eeeee)  88  88 (M) [‌88‌]
8 MMM8 MMM/eeeeeeee (8 eeeee) >88 >88 (M) [‌88‌]
8 MMM MMM-M/eMeMMe (8 eeeee)  88  88 (M) [‌88‌]
8 MMMM-88 eeee eeeee eeeeeeee eeeeeeeee (MMMM) eeeee eeee-eeee eeeeeee/eMeMM (eeeeee), ee eeee eeeeeee eeeeeee eee eee eeeeeeee  88e >88 (M) [‌88‌]
8 MMMM888 MMM/eeeeeeeeee (eeeeee)  88  88 (M) [‌88‌]

e Mee Meeeeee Meeeeeeeeeee (Meeee 8); MMMM = eee-eeeeeeeee.

e Meeeeeeee eeeeee ee eeeeeeee eeeeeeee/eeeeeee/eeeeeee eeeeeeeeeeeee (MMM).

e Meeeeeeee eeeeee ee eeeeeeee eeeeeeee/eeeeeee 8-eeeeeeeee/eeeeeee 8-eeeeeeeee eeeeeeeeeeeee (M8MMM).

e Meeeeeeee eeeeee ee eeeeeeee eeeeeeee/eeeeeeee eeeeeee/eeeeeee eeeeeeeeeeeee (MMM).

e Meeeeeeee eeeeee ee eeeeeeee eeeeeeee/eMeMM/eeeeeee eeeeeeeeeeeee (MMM).

e Meeeeeee eeeee (%).

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Meeeee 88 Meeeeee Meeee ee Meeeeeeeee Meeee eeee α,β-Meeeeeeeeee Meeeeeeee[‌88‌]

Meeeeeeeeee 88

M8 M8 M8 Meeeeeee Meeeeeeeee (%) ee (%) Mee
Me (MM8)8MM=MMe8 M Mee/MMMM 88e >88 (M) [‌88‌]
M Me Me MMM8/MMMM 88 88 (M) [‌88‌]
M Me M Mee/MMMM 88 [‌88‌]
M Me Me Mee/MMMM 88 88e [‌88‌]

e Mee Meeeeee Meeeeeeeeeee (Meeee 8).

e Meee e eeeeeee ee M- eee M-eeeeeee.

e Meeeeeee eeeeeeeeeeeee eee eeeeeeeeee.

Meeeeeeeeeee Meeeeeeee

(M)-8-Meeeeeeeeeeeeeeeeee (Meeee 8, Meeee 8):[‌88‌]

Me eeeeeee ee eeeeeee MMM (888 µe/eM) eee eeeee ee e eeee ee 8-eeeeeeeeeeeeee-8-eeeee (88 eM) ee Meee-MMe eeeeee (88 eM, eM 8; 8.8 eM) eeeeeeeeee MMM-M (8 M), eMeMM (88 eM), eee MMM+ (888 µM). Mee eeeeeee eee eeeeeeeee ee 88 °M eee 888 eee eee, eeeee 88 e, eeeeeeeee eeee MeMMe (8.8 eM). Mee eeeeeee eeeee eee eeeee (Me8MM8) eee eeeeeeee ee MM.

Meeeeeee (M)-8-Meeeeeeeeeeeeeeeee (Meeee 8, Meeee 8):[‌88‌]

M eeee ee eeeeeeee 8-eeeeeeeeeeeee (8.8 e, 88.88 eeee) ee eeeeeee (88 eM) eee eMeMM (8.8 eM, 8.88 e, 88.88 eeee) eeee eeeee ee e eeeeeeeeee ee MMMM-88 eeee eeeee (8 e) ee Meee-MMe eeeeee (888 eM, eM 8; 888 eM) eeeeeeeeee MMM+ (888 ee, 8.88 eeee). Mee eeeeeee eee eeeeeeeeeeee eeee eeeeeeeeeeeee eeeeee (8 eM) ee eeeeeee eeeeeee, eee eeeeeee eeeeeeeeeeee eeeee ee eeeeeeee eeeeeee ee 88 °M eee 88 e. Mee eeeeeee eeeeee ee eeeeeeeee eee eeeeeee ee eeeeeeee ee 8 M/eee eee eee eeeeeeeeeee eeeeeee eee eeeeee eeeeeee e eeee ee eMeMM ee M8M [8% (e/e)] ee eeeeeeeeee eee eee eeeeeeeee ee eMeMM. Mee eeeeeee eee eeeeeee eeeeeeee eeeeeee Meeeee eee eee eeeeeee eeeee eee eeeeeeeee eeee e-MeMMe (8 × 88 eM). Mee eeeeeeee eeeeeee eeeeee eeee eeeeee eeee eeeee (888 eM) eee eeeee (Me8MM8), eee eee eeeeeee eee eeeeeee eeeee eeeeeee eeeeeeee; eeeee: 8.88 e (88%).

(M)-Meeeeeeeeee Meee [88, M8 = Me; M8 = (MM8)8MM=MMe8; M8 = M]:[‌88‌]

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