0 Hits

  • Previous / Next

You are using Science Of Synthesis as a Guest.
Please login to access the full content or check if you have access via Encoded Self-Assembling (Dynamic) Chemical Libraries

DOI: 10.1055/sos-SD-241-00223

Prati, L.; Lucaroni, L.; Cazzamalli, S.; Neri, D.; Oehler, S.Science of Synthesis: DNA-Encoded Libraries, (20241519.

General Introduction

DNA-encoded chemical libraries (DELs) are large collections of compounds, individually linked to specific DNA tags, serving as unique amplifiable identification barcodes. DELs facilitate the synthesis and screening of large numbers of compounds at a time against proteins of pharmaceutical interest.[‌1‌] DELs can be classified as single- and dual-pharmacophore libraries, depending on whether the display of the building blocks occurs at the extremities of one or two complementary strands.

Meeeeee eeee-eeeeeeeeee eeeeeeee (MMMM) eeeeeeeee, eeeeeeeeee ee 8888 ee Meee eee eeeeeeeeee, eee eee eeeee eeeeeee ee eeee-eeeeeeeeeeeee MMMe.[‌8‌,‌8‌] Me eee MMMM eeeeee, eee eeeeeeeeeee eeee ee eeeeeeeeee eeeeeeee eeeeeeeeeeeee eeeeeeeeeeeeeeee (eeeee “eeeeeeeeeeee”) eee eeeeeeeeee ee eeeee ee eeeeeeee eeeeee eeeeeeeeeeeeee. Meeee eeee eeeee eeeeee, eee MMMM eeeeeeeee eeeeeeee eee eeee eeeeeeeeeeee eeeeeeeee eee eeeeeeeee ee eeeeee eee eeeeeeee eee eeeeeeeee ee eeeee eeeeeeeeeeeee eeeeeeeeeee ee eeeeeeeee.[‌8‌‌8‌] Mee eeeeee MMMM eeeeeeee eeeeee ee eee eee ee eeeeeeeeeeeeeee eeeeeeeee eeeee eeeeeee eeeeee eeeeeeeee (e-eeeeeee) eeeeeeee ee eee eeeeeeee eeeeee eeeeee eee eee eeeeeeee eeeeee. Mee eee ee eee e-eeeeee, eeeeeeee ee Meeeee eeee-ee, eeeeee eee eeeeeeee eeeeeeeeeeeee eeeeeeeee ee eeeeeee eeeeeeeeeeee. Me eeeee ee Meeeee 8, eeeeeeeeee eeeeeee ee eeeeeeeeeeee M eee M eee eeeeeeeeeeeeeee eeeeeeeee eeeeee ee eee eeeeeeee ee ee eeeeee eeee (e-eeeeee). Me eee eeee eeee, eee Meee M ee eeeeeeeeeee eeee eee eeeeeeeeeeeee eeeeee ee Meeeee eeee-ee eeeeeeee, eeeeeee ee eee eeeee MMMM.[‌8‌] Meeeeeeeeeeee, eee eeeeeeeeeeeeee ee eeee eeeeeee eeeeeee, eeeee eeeeee eee eeeeeeee ee eeee eeee eee eee ee eeeeeeee eeeeeeee eeeeee ee eeee eeeeee, ee eeee eeeeeeeeeee.[‌8‌] Meeeeeeee eeeeeeee ee eeeeeee MMMM eeeeeee eee eeeeeeeee ee Meeeee 8 (eeeee “MMMM 8+8” eee eeeeee “MMMM+”).[‌8‌] Meeeeeeee ee MMMM eeeeeeeee eee eeeeeeee ee eee eeeeeeeeeeeeee ee eeeeeee eeeeeee eeeeee eeeeeeee ee eeeeeeeeee eeeeeeee, eeeeeeeee eeeeeeee eeeeeeeee MM (MMMM), eeeee-8-eeee eeeeeeeeeeee (MMM), eee e-Mee M-eeeeeeee eeeeee (MMM8).[‌8‌,‌8‌] Mee 8+8 eeeeee eee ee eee eeeeeeeee ee ee eeeeeeee-eeeeeee MMM eeeeee eeee e 88-eeee eeeeeeeee eeeeeeee eeeeeeee ee eee eeeeeee eee.[‌88‌]

Meeeee 8 Meeeeee Meeeee eee eee Meeeeeeee ee ee Meeeeee Meee-Meeeeeeeee Meeeeeee Meeeeee eee Meeeeeee ee MMMM Meeeeee[‌8‌,‌8‌]

MMM-Meeeeee Meeeeee Meeeeeeee

MMM-eeeeeee eeeeeee eeeeeeeee eeee eeee eeeeeeeee eee eee eeee-eeeeeee ee eeeeeeee eeeeee eeeeeeeee ee eeeeee eeeeeee eeee-eeeeeeeeee eeeeeeee (MMMM) eeeeeeeee.[‌8‌,‌88‌] Mee eee ee eeeee eeeeeeeeeeeee MMM eeeeeee (e.e., 8–8 eeee eeeee) eeeeee eee eeeeeeeee ee eeeeeeee eeeeeeeeeeeeee ee eeeeeee eeeeeeeeee eeeeeee, eeeeeee eeeeeeee eeeee eeeeeeeee eee eeeeeeee eeeeeeeeee (Meeeee 8, eeee M).[‌88‌‌88‌] MMM-eeeeeeee eeeeeeeeee eeee eeeeeeeeeee eee eeeeeeeee eee eeeeeeeee ee eeeee eeeeeeeee ee eeeeeee eeeeeeee, eeeeeee ee eee eeeeeeeeee eeeeeeeee ee eeeeeee eeeeeee eeeeeee eeeeeeee ee eeeeeeeeeeeeee eeeeeeee eeee ee eeeeeee 8 ee eeeeeeeeeee eeeeeeee.[‌88‌,‌88‌]

Meeeee eeeeee MMMM eeeeeeeeee, eeeeeee eeeeeeeeeeee eeeeeee eeeeeeee eeeeeeeeeee eeeeee eeeeeeeee eeeeeeeeeee. Meee eeeeeeeeee eeee eee eeeeeee ee eeeeeeee, eee eeeeeeeee ee eeeeeeeeeeee eeeee eeeeeeeeeeeee ee eeeeeeee eeeeeeeee eeeee eeee ee eeeeeeee eeeeeeee eeee eeeee eeeee eeeeeeeee eeeeeeeeee eee eeeeeeeeeeeee.[‌88‌] Meeeeeeee eeeeeeeeee eeee eeee eeeeeeeee ee eeeeeee eeeeeeeee eeeeeeeeeeeeee eeee eeeeeeeeeee eeeeeeee eeeee, eeeeeeeee eee eee ee eeeee-eeeeeeeeeeee ee eeeeeeeeeee MMM eeeeeeeeee (e.e., M eeeee). Meeeee 8, eeee M eeeeeeeeee eeeeeee eeeeeeeee eeee eeeeeeeeeeee eeeeeeeeeeeee eeeeee, eeeee eee eeeeeeee eee eeeee-eeeeee eeee MM eeeeeeeeeee. Mee eeeeeeee MMM eeeeeeee ee eeeeeeeee ee eee eee ee e eeeeeeee eeeeee eee e eeeee eeeeee eee eeeeeeeeee MMM eeeeeeeeeeeee eee eeeeeeeeee. Meeeee 8, eeee M eeeeeeeeeee eee eeeeeeeeeee eeeeeeee eeeeeeee eeeee ee M-eeeee MMM eeeeeeeeeeeee, ee eeeee eee eeeeeeee MMM eeeeeeee ee eeeeee eeee eeeeeeee ee eeeeeeeee eeeeeeeeeeee.[‌88‌,‌88‌,‌88‌,‌88‌]

Meeeee 8 Meeeeeeee Meeeeeeeeeeeee ee MMM-Meeeeee Meeeeee Meeeeeeee[‌88‌‌88‌,‌88‌,‌88‌]

Meeeee Meeeeeeeee eee MMMM-Meeeeee Meee

Meee eeeeeeeeeeeeee ee eeeeeeeee eee eeeeeeeee eeee MMMM eeeeeeeee eeeeeeeee, eeeeeee ee eeeeeeee eeeeeeeee eee ee ee eeeeeeeee ee eeeeeeee eeeeeeeeeee eeeeee. Meeeeeeeee eee ee eeeeeeeee ee eee eeeeeeee (ee-MMM) ee eeeeeee (eee-MMM) ee e MMM eee.[‌8‌,‌88‌‌88‌] Meeeeeee eeeeeee eeeeeeeeee eeeeeeeeeeeee eeeeeee eeeeeeeeeeee eeeeeeeeeeee (MM), eeeeee-eeeeee eeeeeeeeeeeee eeeee (MMMMM), eeeeeee eeeeeee eeeeeeeee (MMM) eeeeeeeeeeee ee e MMMeeee eeeeeeeeee, eee eeeeeeeeee eeeeeeeee eeeeeeeeeee (MMM).[‌8‌,‌88‌,‌88‌,‌88‌] Meee eeeee eeeeee eeee eeee-eeeeeeeeeeeee eeeeeeeee eeeeeee ee eeeee ee eeeeeeee eeeeee (eeeeeeeee). Meeeee ee-eeeeeeeee ee eee eeeeeeee eeeee ee eee eeeeeeeee ee eeeeeeeeeeeee eeeeeee eeeeeeeeeeeee MMM/MMM eeeeeee eeeeeeeeeee eee-eeeeeeeeee eeeeeeeeee. Meee eeeeeeeeee ee “eee-MMM” eee eeeeeeeeee, ee-eeeeeeeee ee eeeeeeee eeeee eeeeeeee eee eee ee e eeeeeeee eeeeee ee eeeeeee eee MMM eee eeee eeeee eee eeeeeeeee eeeeeeee ee eee MMMM eeeeee.[‌8‌,‌8‌,‌88‌] Me eee eeeeee eee e eeeeee eeeeee ee eee eeeeeeee eeeeeee eee eeeeee eeeeeee, eeeeeeeeeeee eeeeeeeeee eeeeeeeee eeee ee ee eeeeeeeeee.[‌88‌,‌88‌]

Meeeeeeeeee ee MMMM Meeeeeeee ee eee Meeeeeeeeeeeee Meeeeeeeeee ee Meeee-Meeeeeee Meeeeeeeeeee

MMMM eeeeeeeee eee eeeeeee eeeeeeeee eeee eeee eeeeeeeeeeee eeeeeeeeeee eee ee eeee eeeeee eeeeeeeee eee eee eeeeeeee-eeeeeeeeee eeeeeeeeeeee.[‌8‌,‌8‌,‌88‌] Me eeeeeee ee eeeeeeeeee ee eeeeeeee eeeeeeeee, eeeee-eeeeeeee eeeeeee eee eeeeeeeeeeee eeeee eeeeeeeeee ee eeeeeeeee eeeeeeee eee eee eeeeeeeeeee ee eeeee-eeeeeeee eeeeeeeeeeee, eeeeeeeee eeeee eeeeeeee–eeee eeeeeeeeee (MMMMe) eee eeeeeeeeeee eeeeeeeeeeee.[‌88‌,‌88‌] Meeeeee eeee ee eeee ee eee eeeeee eeeeeee eeee eeee eeeeeeee (eeeeee eeeee ee eee-eeeeeeeee eeeeeeeeeeeee eeeee) ee eeeeee eee eeeeeeeee eeeeeeeeeeee eee eeeeee ee eee eeeeeeee ee eee eeeeeee eeee.[‌88‌] Mee eeeeeee, eeeeeeeeeeeee (MMM), e eeee-eeeeeeeeeeeee eeeeee ee eeeeeeee eeeeeeeee MM (MMMM), eee eeee eeeeeeee-eeeeeee ee eee eee ee ee MMMM eeeeeee. Meeeeeeeeeeee-eeee (MMM+), e eeeeee eeee eee eeeeeeee eeeeee ee eeeeeeee eeeeeeeeee MMMM eeeeeeeeeee, eeeeeeee eeeeee eeeeeeee ee MMMM eee eeeeeeee eeeee-eeeeeeeee eeeeeeeeeee.[‌8‌,‌88‌] Meee e eeee-eeeeeeee eeeeee ee eeeeeeeee, eee eeeeeee eeeeeeee ee MMMMe eeeeeee eee eee ee eeeeeeeee eeeeeee eee eeeeeee eeeeeeee eee eeeeeeee eeeeeeeeee eee eeeeeeeeeee eeeeeeeeeeee.[‌88‌,‌88‌] Me Meeeeee, ee eeeeeeee eee eeeeeeeee ee e MMMM eeeee ee MMM+ eeeee ee eeeeeeeee ee e eeeeeeeee eeeeeee ee e eeeeeeeee eeeeee.